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The potential of centrifugal partition extraction (CPE) combined with the ion-pair (IP) extraction mode to simultaneously extract and purify natural ionized saponins from licorice is presented in this work.
A method based on the ion-pair extraction mode was developed to extract glycyrrhizin (GL), a biologically active ionic saponin naturally present in licorice (Glycyrrhiza glabra L., Fabaceae) roots.
Since the traditional preparation involved steeping the roots peels in water, alcoholic beverage, or chewing root peels, we first screened for antimicrobial activity using water and methanolic crude extract to mimic this traditional extraction mode.
Different parameters affecting extraction efficiency such as extraction mode, fiber coating and temperature were studied.
High throughput, with six samples running simultaneously in a highly automated system working in dynamic extraction mode, has been achieved.
In DC extraction mode (leaky mode) ion currents in the order of hundreds of pA are detected.
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Static and dynamic extraction modes were compared.
Also, feedback evaluation strategy for design of texture extraction modes, needed by self-adaptive texture extraction mechanism, is provided.
In order to overcome this issue, a new optimization approach was proposed based on a combination of extraction modes and extraction temperatures in a single extraction procedure.
Under the optimum extraction conditions, good linearity was obtained by all of three SBSE extraction modes with correlation coefficient (R) higher than 0.9971.
The two extraction modes have common and uncommon features, which make the use of one or the other more suitable for a given type of sample extraction.
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