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Under optimum conditions, 5 mL of wine were extracted with an extraction mixture consisting of 1.43 mL of acetone, and 173 μL of chloroform at room temperature.
After extraction, mixture was filtrated and obtained extracts were recovered and used for determination of total phenolic compounds, total flavonoid and tannin content, as well as antioxidant activity.
The apricot pomace samples (2 g) were individually extracted three times with 20 mL of extraction mixture (hydrochloric acid/methanol/water in the ratio of 1 80 19) at 40 °C for 30 min in an ultrasonic bath [16].
The extractive systems studied in this work were HTE, DEx, conventional extraction, mixture extraction (MEx), and DSD (new process).
The dried aqueous extracts from 100 μl of the aqueous phase of the extraction mixture were derivatised with methoxyamine hydrochloride in pyridine (20 mg ml-1, 30 μl, 17 hr, 22°C) and silylated using MSTFA (30 μl, 1 hr, 22°C) [ 33].
After overnight extraction at -20°C, solids were separated by centrifugation at 12000 × g for 10 min at 4°C using a Centrikon T-124 centrifuge with an A8.24 rotor (Kontron Instruments, Cumbernauld, United Kingdom) and re-extracted for 1 h with an additional 4 mL of extraction mixture.
Similar(8)
Central composite design "2n + star" was used in order to optimise the following extraction parameters: number of extractions, composition of the extractant mixture, extraction time and extraction temperature.
The extraction conditions such as extractant mixture composition, extraction time and extractions number, were optimized and the need of an extract freezing step previous to SPME is discussed.
The whole-plot extraction mixtures contained varying proportions of ethanol, ethyl acetate and dichloromethane in a simplex centroid design.
The highest antioxidant activities by the DPPH and ABTS methods were obtained using extraction mixtures containing at least 75 wt% water.
The design is applied to the simultaneous optimization of both mobile phase chromatographic mixtures and extraction mixtures for the Baccharis milleflora (Less).
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