Sentence examples for extraction mix from inspiring English sources

Subsequently, 15 ml of chloroform was added into the extraction mix, and the extraction mixture was mixed at room temperature for 15 min; the mixture was centrifuged at 5,000 × g for 15 min at 4°C.

PLE using ethanol as solvent at 10.7 MPa (50 °C and 100 °C) after 5 cycles of 5 min extraction (mixing in the extraction cell the sample with sand in a ratio 1 4) yielded extracts with respectively 5 and 2.9% sterols.

The overall workflow included SPE extraction, mixing, trapping, and LC-MS.

The main PCR drawback is that DNA or RNA extraction mixes tumour and non-tumour cells and can lead to tumour cells dilution with risk of false negative.

4. Pre-condition the RNA purification column using 250 μL of conditioning buffer and incubate for 5 min at room temp. 5. Centrifuge the column at 16,000× g for 1 min. 6. Pipette 30 μL of 70% EtOH into the cell extract from RNA extraction and mix by pipetting up and down; do not centrifuge.

Another sample was obtained by mixing the cells of both bacteria prior to RNA extraction (Cell Mix).

The pellets were resuspended in a nuclear extraction buffer mix (Thermo Scientific Inc., Rockford, USA) and proceeded to generate nuclear extracts, as described in the manufacturer's instructions.

The supernatants were saved as cytosolic extracts and stored at −80 °C, whereas the pellets were resuspended in 100- μl extraction buffer mix containing DTT and protease inhibitor and saved as mitochondrial fractions.

(f) Extraction Solvent: Mix 700 mL Acetone, 295 mL Water and 5 mL Acetic Acid.

(a) Water: High purity deionized water, filtered through a 0.45 µm or smaller pore filter (b) Acetonitrile: HPLC grade (c) Hexane: HPLC grade (d) Acetone: HPLC grade (e) Acetic Acid: Glacial (f) Extraction Solvent: Mix 700 mL Acetone, 295 mL Water and 5 mL Acetic Acid (g) Mobile Phase A: 0.2% Acetic Acid in Water.

After the proper treatment, cells were washed with PBS and resuspended with 100 ul of 1x Cytosol Extraction Buffer Mix containing DTT and protease inhibitors.