Sentence examples for extraction method following from inspiring English sources

Tumor tissue portions collected in RNA later (Ambion, Austin, TX, USA), were used for DNA isolation by phenol chloroform extraction method, following standard protocol [ 23].

RNA was extracted from scaffolds after mechanical disruption with grinding pestles, using a standard chloroform isopropanol extraction method following manufacturer's instructions (Invitrogen).

This modified protocol allows higher protein recovery yield, while producing protein patterns comparable to those obtained by other extraction methods, following SDS-PAGE and Coomassie or Ponceau staining.

Total-RNA was extracted using the TRIzol extraction method (Invitrogen) following the manufacturers tissue preparation protocol, except for an extended incubation time of 15 min in the phase separation step to maximize RNA yield.

Total doxorubicin was measured by a high-performance liquid chromatography (HPLC)/fluorescence method following liposome dispersion with Triton-X and on-line plasma extraction.

The benefits of our feature extraction method include the following.

The concentration of small PGs present in normal and aneurysmal aortas was determined by biochemical methods following extraction of the tissues with guanidine hydrochloride and treatment with collagenase/elastase, isolation by ion-exchange and gel chromatographies and identification by Western blotting.

Since the structure of this virus has been studied in detail by cryoEM methods, following extraction from cells and vitrification [11, 12], we reasoned that these published findings would provide us with useful reference data to facilitate assessment of the quality of data from the multi-tilt strategy applied to stained and plastic-embedded samples described here.

DTG (MW = 419.38) and DTG-Gluc (MW = 594.51) concentrations in plasma were determined by validated, high performance liquid chromatographic/tandem mass spectrometric methods following extraction with acetonitrile.

Next, to determine whether to use beads for the EFAE or HTEM method, sample extraction was performed using both methods following the procedures described above, and changes in the identification scores were evaluated.

The Fe fractionation using the Tessier consecutive extraction method indicated the following order of Fe distribution and concentration in different soil fractions: RE > FM > OM > CA > EX.