Exact(2)
DPGS performed all fungal culturing, DNA extraction, marker design and validation, PCR, sequencing, alignment, and analyses and drafted the manuscript.
DNA extraction, marker amplification by polymerase chain reaction, and marker display by electrophoresis with nondenatured polyacrylamide gel were performed using the previously described methods (Gu et al. 2004a).
Similar(58)
In this study, maize seeds from two types of hybrid populations were used for DNA extraction and marker analysis: biparental crosses and three-way crosses.
The DNA extraction and marker data were generated as in [[ 15]].
DNA extraction and marker genotyping were already described in Adam-Blondon et al. [ 28].
For further analysis of ions, the negative mode S-plot was chosen for extraction of markers (Fig. 2D).
In other words, external marker extraction is independent of the extracted internal markers and this will improve the robustness of our detection method.
Results also demonstrated that the extraction efficiencies of marker compounds increased when the proportion of the herb containing them was increased and decreased in proportion to amounts of herbs added.
According to Table 2, marker extraction and postprocessing are two time-consuming phases.
Although using markers introduces knowledge about the buildings and their surroundings, how to automatically set the thresholds for marker extraction is still a problem.
Jung et al. [16] proposed a H-minima transform-based marker extraction method that segments cell nuclei in microscopic images by marked watershed algorithm.
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