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There's a disturbing overlay between the regions in India, where natural resource extraction is concentrated, and the areas dominated by a deadly "Maoist" insurgency that has cost the Indian state effective control of much of its territory -- in states like Jharkhand, Chhattisgarh, Orissa, and Madhya Pradesh.
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The EtOAc layer together with the mycelium extraction was concentrated under reduced pressure to afford a crude extract (6.0 g).
The extraction was concentrated under pressure to remove organic solvent and then dissolve in water and partition with EtOAc for four times to give a mycelium-originated EtOAc layer (183 g).
After Soxhlet extraction, each extract was concentrated using an automated nitrogen evaporation system (Turbo Vap II, Zymark Inc).
The solid-phase extraction (SPE) extract was concentrated and then cleaned by passing through a Florisil-H2SO4/silica gel column.
The menstrums collected from each sequential re-extraction were concentrated and blended into a semi-solid paste.
The extraction was done with aqueous ethanol (30 : 70) mixture by hot Soxhlet extraction method and the extract was concentrated in a rotary evaporator under reduced pressure, yielding 720 g (36%) of crude hydroalcoholic extract (HAPA).
Up to 2 g of tissue or 2 mL of urine was taken for extraction, and the final extract was concentrated to as low as 200 μL to get responses above the LOQ range.
The extracts were concentrated (1 100) in double-distilled water to allow extraction of active compound onto the C18 cartridge.
The extract was concentrated using rotary evaporator (BUCHI, R-200 model, Germany) and the extraction yield was estimated from the final extract concentrate using Equation (1).
The extracts were concentrated and strained through a 0.45 μm filter, and the entire extraction process repeated twice.
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