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In brief, The K562 cells were lysed on ice for 15 min using nuclei buffer to get nuclei.Nuclear extraction (Halo nuclei) were prepared by extracting nuclei with halo buffer and isolated by centrifugation through a glycerol step-gradient.
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After extraction, the halos were pelleted at 1000 g for 5 min at 4°C then washed gently in REact® 3 restriction buffer (Invitrogen, Carlsbad, CA, USA) on a rocker platform for 20 min at room temperature then centrifuged at 1000 g for 5 min at 4°C then the supernatant discarded.
The halo extraction may provide both the collimation for the collider and external beams for fixed target physics.
In this case the vertical deflection of about 100 μrad or even smaller in a bent crystal may be sufficient for the collider beam halo extraction.
The optimal parameters of the crystal deflector and the extraction efficiency of a natural collider beam halo were estimated by simulation.
For example, fluorescence in situ hybridization studies of nuclear halos generated after high-salt extraction demonstrated the localization of several active genes on the nuclear matrix, while inactive gene loci were found in the extended DNA loops of the halo [48].
Then glucuronide metabolites from plasma were separated by micro-liquid chromatography coupled with tandem mass spectrometry on a HALO C18 (50 × 0.3 mm, 2.7 μm, 90 Å) column, after solid-phase extraction.
A halo.
Pocahontas meets Halo.
His halo has slipped.
He preferred playing Halo.
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