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All cotton swabs, pipettes, and pipetted solvents of one diffusion cell were collected in one vessel and extracted in the corresponding extraction fluid (water for the extraction of caffeine and 100%% ethanol for the extraction of testosterone).
CO2 was used as the extraction fluid.
Engineered geothermal systems (EGS) may utilise carbon dioxide as a heat extraction fluid instead of water.
The factors used were temperature of the extraction chamber (40 and 70 °C), pressure of the extraction fluid (25 and 45 MPa), and modifier concentration (5 and 15%).
Surface coating occurred through interaction of the negatively charged, nascent PLGA MP with the polycationic chitosan, which was dissolved in the aqueous extraction fluid.
A Plackett Burman design was employed to evaluate the influence of those parameters to be controlled in SFE, such as pressure, temperature, static and dynamic time, restrictor temperature and volume of modifier while CO2 was used as the extraction fluid.
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The intrinsic cytotoxicity of extraction fluids of composite films was investigated by MTT assay using Rat osteoblastic cells (ROS cells).
Volumes of extraction fluids and aliquots of receptor media at various time points were calculated by subtraction of net weights from filled weights of all vials.
The detection limits were below 10 ng/L for Ag, Cd, Sb, Tl, and Pb, and below 1 μg/L for Cr, Fe, Ni, Cu, Zn, and As in the extraction fluids.
Following extraction, fluids were removed, tissues were rinsed with 1 ml guanidine HCL and rinses and extracts were combined.
The proximal shaft is connected to vacuum allowing for the extraction of fluid.
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