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During extraction, ethanol molecules entered into algal cell and extracted intra-cellular lipids and then backed to solvent bulk.
Regarding to solvent extraction, ethanol was used by manually stirring and then by Ultrasound-Assisted Solvent Extraction (UASE).
The 3D shows the response surface plot as a function of temperature (X 1 ) versus time (X 2 ) at fixed extraction ethanol concentration (80 20) as shown in Fig. 2a.
Different strategies of decontamination processes to reduce polycyclic aromatic hydrocarbon (PAH) levels in fish oils, such as solvent extraction (ethanol) and adsorbent extraction using commercially available (activated carbon) and sustainable adsorbents (mussel shell and wood ashes), were compared.
Total RNA from embryos, third instar larvae and adult flies of wild-type flies was isolated by lysis and homogenization in TriZOL (Invitrogen), followed by chloroform/isopropanol extraction, ethanol precipitation, and resuspension in DEPC-water.
The DNA was recovered from immunoprecipitated material by proteinase K treatment at 55°C for 1 h followed by phenol/chloroform (1∶1) extraction, ethanol precipitation, and resuspension into 50 µl of nuclease-free water.
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ChIP DNA was purified by phenol-chloroform isoamylalcohol extraction, ethanol-precipitated with 10 µl acrylamide carrier and dissolved in 10 µl MilliQ water.
DNA was then purified by phenol/chloroform extraction, ethanol-precipitated, and resuspended in 75 μl of TE.
The product was purified by phenol/chloroform extraction, ethanol-precipitated, and resuspended in 200 μl of 1× reaction buffer (30 m m sodium acetate, pH 4.5, 50 m m NaCl, 1 m m ZnCl2).
R. anatipestifer genomic DNA was extracted and purified via proteinase K treatment, multiple phenol extractions, ethanol precipitation, and spooling.
DNA was extracted via phenol extraction and ethanol precipitation.
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