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To achieve these goals, during method development, different options were evaluated to optimize sample extraction, detection parameters, and chromatography.
Each laboratory used slightly different methods, which included different extraction, detection and analytical methods.
However, several problems are encountered during extraction, detection and identification of CWPs by proteomic approaches [ 16].
It technically consists of three well-defined phases: extraction, detection on the LightCycler 2.0 machine and data analysis.
HPV extraction, detection and genotyping were carried out from S-Dry and S-Wet specimens using the Cobas 4800 (Roche Diagnostics International Ltd Forrenstrasse 2 CH-6343 Rotkreuz, Switzerland) HPV test according to the manufacturer's recommendations.
The method comprises an acidic acetonitrile extraction, detection with ultra-high-pressure liquid chromatography coupled to electrospray ionisation time-of-flight mass spectrometry and statistical evaluation using a metabolomics approach comparing suspected contaminated food with uncontaminated foods.
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The principal tasks are high-level feature extraction, copy detection, and surveillance event detection.
The whole CAD pipeline can be outlined in four stages: VM extraction, IPC detection, FP reduction and detection presentation, as shown in Figure 1.
Our positive controls were infected mosquitoes, showing that our DNA extraction and detection methods are optimized for in vivo detection despite using an extraction protocol different from those used in the original publications.
Extraction and detection procedures were designed and optimized to achieve detection in a respectable time frame, at a reasonable cost, and with a high throughput capacity.
These are road extraction, car detection, and car tracking (see also Fig. 4).
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