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The detailed procedure is described subsequently.
The two steps are described subsequently.
Detailed laboratory methods are described subsequently.
The two sections were subsequently processed independently, with one section used for RNA extraction and the other used for total protein extraction (described below).
The extraction method utilised was MEM extraction, previously described, with minor modifications (Barbi et al, 1996).
The yolk sac membranes were excised and washed with ice-cold phosphate-buffered saline (PBS) and subsequently used for RNA extraction as described below.
The protein extraction technique employed was similar to the extraction technique described by [ 23].
Subsequently, about 500 mg bone powder was used for a silica-base DNA extraction as described [18].
Enzyme extraction is described further on.
Joseph Nielsen, Oregrown's head extraction engineer, described delta-8's effect as "a solid punch".
The hybridization and features extraction were described in [59].
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