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The ASL-FAIR technique is suitable for absolute blood flow quantification in the rat brain when using a one-compartment model including a suitable extraction correction for data evaluation.
Below about 2 mL/min/mL, an extraction correction can be performed with the help of Equation 14 using the fitted values of PS and N (but note that N is directly proportional to the chosen value of V d ·ρ a : if a different value were chosen for this product N would have to be adjusted accordingly).
Scattered throughout the analytical batch, they facilitate the assessment of metabolite recovery after extraction, correction of instrument drift, analyst variation, and data cleaning [ 37].
Analysis of each spot was made using Feature Extraction software 6.1 (Agilent Technologies), using the default/recommended parameters for the scanning, griding, extraction, correction and normalisation.
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The reduction steps carried out by the PLP include flat-fielding, background removal, order extraction, distortion correction, wavelength calibration, and telluric correction using spectra of A type standard stars.
As the spectrograph has no moving parts, the PLP automatically reduces the data using predefined functions for the processes of order extraction, distortion correction, and wavelength calibration.
Details about the process of information extraction and correction are available upon request.
ID standards are added prior to extraction, allowing correction for recovery over the multistage clean-up procedures.
Pre-processing consisted of brain extraction, motion correction, spatial smoothing (Gaussian kernel, full width at half maximum = 5 mm), high-pass filtering (cut-off = 60 s) and pre-whitening.
Blood oxygen level dependent (BOLD) images were preprocessed in AFNI software with standard steps, including brain extraction, motion correction, spatial smoothing (8 mm full width at half maximum), and voxelwise time course normalization to percent of mean signal level.
To report an unusual complication of accommodative intraocular lens (IOL) implantation after clear lens extraction for hyperopia correction.
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