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Next, the solvent mixture for FA derivatization and extraction, consisting of heptane (400 μL), and the methylation mixture (680 μL, methanol:benzene:DMP:sulfuric acid = 39 20 5 2, by vol) was added to the amber vial, which was then placed in a water bath/shaker (BF-45SB, Biofree Co., Ltd., Seoul, Korea) for 2 h at 80 °C.
The membrane extraction with 1-pentyl-3-methylimidazolium bromide coated titanium dioxide ([PMIM]Br@TiO2) sorbent used in this research is a two-phase supported membrane extraction consisting of an aqueous (donor phase), and n-octanol/nano [PMIM]Br@TiO2 (acceptor phase) system operated in direct immersion sampling mode.
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The second stage, OFDM feature extraction, consists of several steps.
The conventional Mel-cepstral feature extraction consists of the following steps.
Quotation Extraction consists of identifying quotations and their author from a text [90].
ECG feature extraction consists of determining a set of discriminant features to represent ECG data to achieve strong classification performance.
The electrochemical measurements of Li+ insertion and extraction consisted of cyclic voltammetry, chronopotentiometry and potentiostatic intermittent titration technique.
The use of nonionic surfactants in liquid liquid extraction consists of a two-phase process without the presence of an organic solvent.
The system that allowed for the highest extraction consisted of 15% (w/w) PEG 3350-123350-12%phosphate, at pH 7.0.
In addition, the RSM analysis predicted that the optimum conditions for extraction consisted of a solid liquid ratio of 20, a time of 60 min, and a temperature of 60 °C.
Each DNA extraction consisted of a single megagametophyte.
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