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The optimal buffer compositions were experimentally identified based on the evaluation of extraction consistency and efficiency from rat livers, with consideration that all buffer components can be completely removed later in the precipitation step.
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Orietta Zaniolo, PharmD, helped in data extraction and the consistency check.
This result indicated a low consistency in extraction among replicate samples.
However, both authors first piloted the data extraction form to ensure consistency in the extraction process.
The classification was developed as we went along with data extraction, and reassessed for consistency after completion.
The form will be piloted on a sample of studies external to the review to ensure consistency of extraction between authors.
After a check had been made for consistency of extraction across two included studies, two reviewers (JL, JR) each independently coded the results data for all papers against the a priori concepts derived from the relevant conceptual model.
This extraction method allowed for consistency across articles and maintained homogenous definitions throughout the process; however, while there may be potential for bias due to extraction by a single reviewer, both authors met throughout the extraction process to clarify interpretations of extracted data.
LC/MS analyses of the different batches of MeOHGCM6 extract were performed to ensure reproducibility and consistency of the extraction methods.
There was little consistency among extractions made from different parts of the same plant.
Thirty per cent of the extractions were done in duplicate as an initial calibration process to maximise the consistency of data extraction across reviewers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com