The DNA extraction was automated performed and the STR loci was PCR amplified using PP17 kit.
As continuous recording leads rapidly to the accumulation of large amounts of data, parameter extraction and automated processing become crucial.
Brain extraction and automated tissue segmentation were based on previously described methods [ 39, 40].
Liquid handling before extraction was automated using the 215 Liquid Handler (Gilson Inc., Middleton, WI), and SPE was automated using the Rapid Trace SPE workstation (Zymark, Hopkinton, MA).
Alcohol Insoluble Materials (AIMs) were extracted using an automated extraction method with accelerated solvent extraction unit ASE® 350 (DIONEX, CA, USA).
For those sites not routinely using data extraction tools, the automated data extraction tool will be temporarily installed for data collection purposes only and then uninstalled that same day.
For the screening of PCR-based markers over the whole population, leaf tissue samples were collected from all individuals and DNA extracted using an automated extraction protocol.
Total genomic DNA was extracted by using phenol-chloroform extraction using the Autogen automated DNA extraction robot (AutoGen Inc., Holliston, MA) and eluted in 50 μL of buffer in a 96-well plate format.
An aliquot of 250 μl was used for DNA extraction on the Abbott m24 sp automated extraction platform (Abbott, Maidenhead, United Kingdom) according to the manufacturer's instructions.
For British Columbia and Ontario, 200 µL and 250 µL, respectively, of sample were extracted using the NucliSENS easyMag automated extraction system and then treated with Turbo DNase ("post-DNase" treatment).
