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Total RNA extraction and single strand cDNA synthesis were performed as previously reported [ 85, 39].
(B ) Total lymph node cells were analyzed for the indicated phospho-proteins either immediately after extraction and single cell suspension generation (0') or after a 30 min rest period in PBS at 37° (37°).
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The method combines the advantages of dynamic microwave-assisted extraction and single-drop microextraction, which could greatly simplify the operation and reduce the whole pretreatment time.
The rest of the article is organized as follows: We first describe the various cepstral, filterbank feature extraction and single-channel dereverberation techniques considered in this work in Section 2. In Section 3, we provide a brief description on how to train and extract low-dimensional i-vectors from the training and test recordings.
DNA extractions and single length telomere analysis (STELA) reactions at the 17p and XpYp telomeres were carried out as described previously and in full in supporting information (Text S3) [41].
The price for extractions and single surface, one-tooth fillings for out of pocket payments (OOP) from the facilities were reported in Tanzanian shillings (Tshs).
Each subject donated 5 ml of blood for genomic DNA extraction and a single spot urine sample was collected from each subject on the same day as the semen sample.
A total of 9,024 clones were randomly selected for plasmid extraction and subsequent single-pass sequencing from the 5' and 3' ends.
A total of 5760 clones were selected for plasmid extraction and subsequent single-pass sequencing from the 5' end.
High-salt buffer was required for LRRK2 extraction, and although a single LRRK2 band was observed in the high-salt fraction for c41-2, c81-8 and c68-7, additionalnal higher-molecular-mass band was apparent in the detergent-soluble fractions.
Transcripts were characterized by gel extraction of PCR products and single molecule sequencing.
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