Genomic DNA was extracted using a conventional proteinase K extraction and a QIAamp DNA Blood Kit (Qiagen, Valencia, CA) following the manufacturer's protocol and stored at −80°C.
Rotavirus dsRNA was obtained from 10% fecal extracts by using a standard phenol-chloroform extraction and a hydroxyapatite purification method (23 ).
One reviewer (MvdD) performed the data extraction and a second reviewer (AE) verified all extracted data.
After nuclei extraction and a 5 minute DNase I digestion the DNA was extracted and real-time PCR was conducted on an ABI 7000 Sequence Detection System.
MR. LONOA'S trip to America included his first visit to a dentist, for an extraction, and a journey by bus to New York and the United Nations.
This method is composed of two sequential processes: a feature extraction and a rule decision.
After plasma deproteinisation, a diethylic extraction and a N-tert.-butyldimethylsilyl-N-methyltrifluoroacetamide derivatisation were performed.
Vidergar, N., Toplak, N. & Kuntner, M. Streamlining DNA barcoding protocols: Automated DNA extraction and a new cox1 primer in arachnid systematics.
A comparison of Soxhlet extraction and a new extraction technique, fluidized-bed extraction, has been conducted.
(His father, who was of German extraction and a troubled soul, split up with his mother when he was young, and he was brought up by her family, the de Blasios — that's his version).
I'd managed to get through the account of a baby's face mangled by an inept midwife in the English countryside, also an ill-fated forceps extraction and a tour of maternity wards corrupted by childbed fever.
