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Subtree extraction, alignment and phylogeny construction was repeated until subtrees comprised a maximum of approximately 200 sequences.
The XCMS program (http://metlin.scripps.edu/download/) was used for peak extraction, alignment, and integration in a visual matrix.
The extraction, alignment, deconvolution, and further processing of raw GC/MS data were principally referred to the previous published protocol [ 35].
We run a NIP extraction alignment procedure as described in more detail in the following, which aims at identifying orthologous and nearby intron positions within the data set.
After an automatic NIP extraction alignment procedure, we obtained 122 NIP regions containing two or more near introns (NIP distance < 50 nt).
The extraction, alignment, deconvolution, and further processing of raw GC/MS data were referred to the protocols of literature [ 47- 49], except the mass window was set to 70 600 m/z.
Similar(53)
Here, tools dedicated to ion chromatogram extraction, time alignment, signal normalization and statistical analysis were used to interpret label-free relative difference between primary human chondrocyte secretomes and dilutions thereof, analyzed successively by LC-MALDI.
AB implemented the ORF extraction and alignment software used, and advised on strategy.
The data-processing procedure commonly includes data reduction, denoising, metabolite extraction, and alignment [ 39 ].
CRT carried out DNA extraction, amplification, alignment, performed all phylogenetic and divergence time analysis, and drafted the manuscript.
Recently, we presented a complete strategy to perform a convenient data extraction and alignment using two-dimensional gas chromatography coupled with mass spectrometry (GCxGC-MS) technology [ 25].
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