In order to achieve the best possible extraction results, a number of parameters that influence the extraction efficiency were investigated and the optimal conditions were employed in the subsequent analyses.
SDS-PAGE and Western blot analysis of sequentially extracted knee cartilage revealed genotype-specific differences in the extraction of a number of proteins that included matrilin-1 to -4, COMP and type IX collagen, which are all known to interact with each other (Budde et al., 2005; Fresquet et al., 2010; Fresquet et al., 2007; Holden et al., 2001; Mann et al., 2004; Wiberg et al., 2003).
Also, the extraction of a number of alternatives may cause a different order of priorities.
The model can also easily be extended to model the extraction of a number of different coffee constituents rather than just a single entity.
It was shown by simulations and by theoretical considerations that conventional regime analysis fails for reactive extraction in a number of regimes, because the conditions of an irreversible reaction and a negligible resistance to mass transfer in the non-reactive phase are generally not fulfilled in reactive extraction.
Given this, we preferred the freeze-thaw aqueous methanol extraction for a number of reasons.
Solid phase extraction has a number of advantages, including reduction in volume of solvents used, simplicity of operation, and the possibility of automation (online coupling with high-performance liquid chromatography (HPLC) or gas chromatography (GC)).
Nascimento et al. [ 20] prepared ethanol extractions of a number of plants and tested these against a range of laboratory and clinical isolates.
Both methods have recently demonstrated the potential to reduce extraction times significantly and increase extraction yields in a number of studies on medicinal plants [8].
We further illustrate the value of the extraction approach through a number of analyses of the extracted information.
