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In order to improve the simultaneous extraction efficiency of the twenty one veterinary drugs, a simplex-centroid design with combinations of the three components of the extracting mixture, i.e. MeOH, ACN and sodium phosphate buffer 10 mmol L 1 pH =3.50, was carried out.
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The herbal extract mixture and the single extracts were provided by Bionorica SE, Germany.
A lower A260/230 nm ratio may be due to phenol contamination in the extracted mixture.
For the in vivo studies the dry extract mixture was suspended in 0.9% saline (w·w− 1).
Open image in new window Fig. 5 FT-IR analysis of a SNPs/Satureja intermedia C.A. Mey extract mixture and b Satureja intermedia aqueous extract.
The extracted mixture of RLs produced in the presence of sodium gluconate was subjected to identification and characterization using LC- ESI -MS.
All or some of the ingredients in the extract mixture may be responsible for the anticancer activity in the additive or synergistic fashions.
Open image in new window Fig. 2 UV-Vis absorption spectra of a Satureja intermedia C. A. Mey and b SNPs/Satureja intermedia extract mixture.
The extracted mixture was then centrifuged at 3000 g for 10 min to collect its supernatant.
Solvent from each extracted mixture was evaporated to dryness using a rotary evaporator under reduce pressure at 40°C.
Khz-cp is an extract mixture from the mycelia of a G. lucidum and P. umbellatus nuclear fusion.
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