Methanol extract (ME), obtained by successive solvent extraction, its most effective liquid-liquid n-butanol fraction (NBF) and the flash column chromatographic sub-fraction (SFI), were evaluated for in vitro α-glucosidase (yeast) and α-amylase (porcine) activity inhibition.
The extraction process yielded 4.08% of the methanol extract (ME), 0.42% of n-hexane fraction (HF) and 3.56% of methanol fraction (MF) (Table 1).
Strains were grown in a base medium composed of 100 g/L malt extract (ME) (Sigma-Aldrich, St . Louis MO, USA).
Then, the methanol extract (ME) was centrifuged at 5000g for 15 min at 35 °C.
Subsequently the organic phases were combined and evaporated to yield 221 mg of oily mycelial crude extract (ME) in total.
The highest level of TPC was detected in the methanol extract (ME), whereas the lowest was observed in the chloroform extract (CE).
In antibacterial assay, the PEE and chloroform extract (CE) showed mild to moderate antibacterial activity against all the bacterial strains except B. cereus while methanol extract (ME) showed no inhibitory effect against any of the tested bacteria.
The effect of nitrogen source on POME fermentation was studied using seven different nitrogen sources, i.e. yeast extract (YE), meat extract (ME), peptone, ammonium chloride (NH4Cl), ammonium sulphate (NH4 2SO4, glutamic acid and urea at 1 % (w/v), respectively.
In the current study, different plant extracts of Gynocardia odorata such as methanol extract (ME), aqueous soluble fraction (AQSF), chloroform soluble fraction (CSF), carbon tetrachloride soluble fraction (CTCSF), and petroleum ether soluble fraction (PESF) were examined for the analysis of thrombolytic, cytotoxic, and erythrocyte membrane-stabilizing activities.
In the present study, we prepared Magnolia officinalis extract (ME) from dried root barks of Magnolia officinalis.
The methanol extract (ME) gave positive reactions for alkaloids, saponins, tannins, flavonoids, resins, steroids, terpenoids and carbohydrates.
