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According to Marques et al. [ 27] water is the best extracting liquid for H. tomentosa roots regarding extractable solids.
The problem of removing the extracting liquid is eliminated.
Later, a central composite design was applied for optimizing the temperature and the composition of the extracting liquid.
The insoluble formazan was extracted with 1.0 ml isopropanol solution (containing 0.04 M HCL; Fisher Scientific, USA), 1 ml per well, at 34°C for additional 15 min. The extracting liquid and cells were harvested and centrifuged at 5,000× G for 10 min. The amount of formazan in the supernatant was estimated by reading the absorbance at 650 and 570.
5 mL protein extracting liquid B (enzyme extracting liquid A plus 5% Triton X-100) was added, the samples were stirred at 4°C for 1 h and then centrifuged at 100,000 × g for 1 h at 4°C.
The extracting liquid was filtered, merged, and then concentrated by rotary evaporator (R-210; Buchi, Essen, Germany) into 1 L liquid extract.
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Laporte, D., Toplis, M. J., Seyler, M. & Devidal, J. L. A new experimental technique for extracting liquids from peridotite at very low degrees of melting: application to partial melting of depleted peridotite.
Press through fine strainer to extract liquid; discard liquid.
Strain over a bowl, pressing lightly to extract liquid.
Strain purée, then press against solids to extract liquid.
2. Strain into a microwave-safe bowl or a saucepan, pressing to extract liquid.
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