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Attempts to quantify functional Fe have been made by extracting in several different extractants, including mild acids and chelating agents.
We therefore utilised a protocol first extracting in CHAPS to release DG from the membrane, followed by a triton extraction step to precipitate the cytoskeleton and associated proteins in the triton insoluble fraction.
In 1960, it began a program to stockpile the helium it was extracting in an underground reservoir near Amarillo, Tex., amassing a debt of $1.3 billion in the process.
After extracting in lysis buffer (7 M urea, 2 M thiourea, and 4 % CHAPS), the concentrations of proteins were measured by 2-D Quant Kit (Amersham Bioscience, USA).
Maximum recovery was obtained by extracting in water at 7 °C, 40 min, 150 rpm and 1 6 solid/liquid ratio.
This technology provides a potential clean method to produce fuel and feedstock material from bitumen, a relatively "dirty" fuel and feedstock oil, in addition to more energy efficient ways of extracting in situ heavy oils.
Similar(49)
To obtain qualitative morphological documentation, two-dimensional pictures were derived from stacks of photographs by extracting in-focus information from z-axis planes to create image mosaics by MetaMorph (Molecular Devices).
Petroleum is also extracted in the area.
Read exclusive extracts in the Guardian this week.
Read exclusive extracts in Sunday's Observer Magazine.
Then water is extracted in one of two ways.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com