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Described briefly, we dissected whole retinas from individual fish and extracted whole RNA from them using Qiagen Qiashredder and RNeasy RNA extraction kits (Valencia, CA).
The pairs of egg sacs are extracted whole, steeped in salt and pressed.
Results show that the correlation of the extracted whole brain (R = 0.80) and the ventricle (R = 0.82) with the experts ground truth is high.
For primary cell cultures, cerebral tissues were extracted whole using fine forceps with connective tissue (dura and arachnoid maters) and vessels removed.
The extracted whole brains were divided equally in the transverse plane using a knife.
Small tumours exhibited a significantly lower uPA activity per mg extracted whole cell protein than larger tumours.
Similar(47)
For immunoblot analysis, we extracted whole-cell proteins from mouse islets in a radioimmunoprecipitation assay (RIPA) buffer [16] using sample preparation procedure A (SPP-A) (detailed in "Materials and Methods").
We behaviorally tested 18 additional fish from each line and extracted whole-brain tissue immediately following a behavioral trial.
We extracted whole-body RNA from 48 samples (2 lines × 3 diets × 2 age classes × 4 replicates) using the NucleoSpin RNA II kit (Macherey & Nagel).
Finally, we extracted whole-cell lysates from the tumor cells and assessed alterations in the levels of these proteins, which were consistent with our in vitro findings.
All children in VIA 7 are identified in the Danish Neonatal Screening Bio-bank, their DNA extracted, whole-genome amplified and genotyped on the Illumina PsychChip, which is a whole-genome SNP array developed in collaboration with the Psychiatric Genetics Consortium PGCC) for genome wide association studies (GWAS).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com