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25 g of ground plant material was extracted successively with distilled H2O in Soxhelt extraction apparatus.
For sequential extraction, fresh leaves of each species (40 g) were freeze-dried, ground and extracted successively with hexane (HEX), dichloromethane (DCM), DCM MeOH (1 1) and MeOH (HmbG Chemicals).
Dried and milled leaf materials were extracted successively with Soxhlet extractor at temperature of 80 °C.
The ethanol extract was concentrated in vacuo and extracted successively with petrol ether (3 x) and CHCl3 mg (3 x).
The ethanol extract was concentrated in vacuo and extracted successively with light petroleum (40 - 60°C) 3 times.
Powdered material (1.2 Kg) was extracted successively with petroleum ether (PET), acetone (ACE) and methanol (MeOH) by using Soxhlet apparatus.
The ethanol extract was concentrated in vacuo and extracted successively with petrol ether (3 x), CHCl3 mg (3 x) and EtOAc (3x).
Two hundred eighty grams of dried and milled plant materials were extracted successively with Soxhlet extractor at temperature of 80 °C.
The total MeOH extracts were concentrated in vacuo, and extracted successively with petroleum ether, ethyl acetate (EtOAc) and n-butanol (n-BuOH).
This is to be expected because these factors are extracted successively, each one accounting for as much of the remaining variance as possible.
The coarsely powdered sample (50 g) was filled in the thimble and extracted successively with petroleum ether, chloroform, ethyl acetate and methanol using a Soxhlet extractor.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com