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Hippocampal tissue was taken, proteins extracted, run on two-dimensional gel electrophoresis and proteins were identified by mass spectrometry methods (MALDI-TOF-TOF and nano-LC-ESI-MS/MS).
Protein was extracted, run on SDS-PAGE, stained and sliced as shown in Figure 1.
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DOI: http://dx.doi.org/10.7554/eLife.01596.009 We next extracted runs from RNP trajectories and analysed their length and velocity.
bPeak one has a retention time and spectrum consistent with that of Vulgaxanthin I, as reported for extracts from beetroot [ 14] and as compared against a beetroot extract run in the same experimental set.
The greatest %RME2 response of 4 8 dilutions of a test chemical or extract run in triplicate was considered detectable if it produced an effect whose average %RME2 was greater than 15% RME2, i.e., more than three standard deviations (3SD, p <0.01, Student's t test) above the mean SC response of a triplicate run for that particular assay.
Conveniently, native mouse IgG in the extract runs at lower molecular weight than ABCA1.
Propolis alcoholic extract ran of the teeth and was not able to form film on tooth surface.
Since inhibition of cell migration is known to contribute to anti-inflammatory activity, enhancement or stimulation of migration by the extract runs counter to the anti-inflammatory activity.
The person later died when the supply of the extract ran out; nevertheless, Züzler continued his attempts to produce a pure extract for a further few years and, in 1911, Hoffman-La Roche assisted Züzler's creation of an experimental laboratory [ 5, 13, 19, 21].
The representative structure for this cluster was extracted from run 4 based on the NMR structure.
From each sample, DNA was extracted and run on the real-time PCR assay.
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