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The surviving resistant clones were isolated, genomic DNA extracted, library inserts recovered by PCR and insert sequences identified by sequence analyses (Figure 3A).
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In collaboration with biologists, this crude extract library has been screened for activity in cell-based and pure enzyme assays designed to identify promising marine natural product lead compounds for the development of drugs.
We identified ursolic acid, a natural pentacyclic triterpenoid that occurs widely in traditional Chinese medicinal herbs, as an inhibitor of PTP1B by screening an extract library of the traditional Chinese medicinal herbs used a diabetes clinic.
Here we describe a pilot scale high-throughput screening (HTS) campaign against the first and second committed steps in the pathway, catalyzed by DXP reductoisomerase (IspC) and MEP cytidylyltransferase (IspD), using compounds present in the commercially available LOPAC1280 library as well as in an in-house natural product extract library.
The extract library is prepared from diverse microbes isolated from variety of ecological units in India.
The metabolite extract library samples were screened for the ability to induce glucose uptake in insulin stimulated differentiated L6 myotubes.
We explored the microbial metabolite extract library in search of compounds that enhance glucose uptake in skeletal muscle cells in presence of insulin.
High throughput screening of diverse microbial metabolite extract library was conducted for detecting glucose uptake inducers in rat skeletal muscle cells.
The group's main responsibility is the isolation and structure elucidation of bioactive compounds from MerLion Pharma's large extract library, which is derived mainly from fungi, bacteria, and plants.
A whole cell extract library was also generated from MCF-7 and followed by direct ultra high-throughput sequencing using Solexa Genome Analyzer platform.
Our extract library was screened for antimalarial activity using a standard high-throughput (HT) SYBR Green I based assay.
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