The remaining insoluble and phenol layers were re-extracted further with double-distilled water and pooled with the above collected aqueous extract.
We conclude that hyperspectral imagery can be used to detect nutrient enrichment across three spatial and at least two temporal scales, and suggest that more quantitative information could be extracted with further research and a greater understanding of physiological and physical mechanisms linking water chemistry, plant properties and spectral imaging characteristics.
The sample was transferred to centrifuge bottles (250 ml) and centrifuged at 6000g for 10 min. The supernatant was collected, and the soil pellet re-extracted with further extraction buffer (50 ml), incubation at 65°C for 10 minutes and centrifuged as above.
The sample was incubated at 65°C for 1 hr, transferred to centrifuge bottles (250 ml) and centrifuged at 6000g for 10 min. The supernatant was collected, and the soil pellet re-extracted with further extraction buffer (100 ml), incubation at 65°C for 10 minutes and centrifugation as above.
From this fingerprint, proteins with biological relevance can be extracted and further examined with other methods and research approaches.
Sequences between open reading frames with Blastp or CDD hits were extracted and further searched with Blastx and Blastn.
Masses with a variable influence of projection score (VIP) > 1 were extracted and further tested with a Wilcoxon-Mann–Whitney-U Test using Matlab R2011b (MathWorks, Ismaning, Germany).
A fourth method (SQ), consisting of standard method S extracted DNA further purified with the Qiagen QIAquick® PCR Purification kit, was included to test if existing DNA stocks could be rescued for use in downstream genotyping experiments.
Polymorphic loci and/or regions of interest in the genomes were extracted and further aligned with ClustalX [ 32].
For each 454-sequencing read, the longest non-masked region was extracted and further trimmed with SeqClean (http://compbio.dfci.harvard.edu/tgi/).edu/tgi/
The insoluble residue was further extracted with 0.5 mol/L KOH with 20 mmol/L NaBH4 for 2 h (shaking, 25°C) centrifuged (10,000 × g, 60 min) and the supernatant filtered.
