To separate the compounds in the ethanol extract fully might require a more polar stationary phase, or the utilisation of a more advanced chromatographic system as well as a more comprehensive spectral library.
For converting to and from this format, we supply the scripts extract-paired-reads.py, interleave-reads.py, and split-paired-reads.py to respectively extract fully paired reads from sequence files, interleave two files containing read pairs, and split an interleaved file into two files containing read pairs.
Furthermore, addition of the full-length xlFANCJ recombinant protein (Supplementary Fig S7D) to a FANCJ-depleted extract fully reversed the persistent stalling at G-quadruplex structures (Fig A and B, right panel) showing that this effect is caused specifically by the depletion of FANCJ.
The scattering powers of single-bounce, double-bounce, volume, helix, and wire scattering components are extracted from fully polarimetric SAR images.
To compare protein expression profiles between WT and the spl5 mutant, total proteins extracted from fully developed leaves with lesions from spl5 and the corresponding leaves from WT were analyzed by 2-DE.
Western blot analysis was performed on membrane preparations extracted from fully differentiated immortalized mouse podocytes [39], [40] mouse mesangial cells [11], mouse fibroblasts [12], rat proximal tubular epithelial cells [13], and rat glomerular endothelial cells [14], as described below.
Contextual information was extracted from fully sequenced prokaryotic genomes using in-house Perl scripts.
Starch was extracted from fully expanded leaves of all the mutants and the wild type, and analyzed in the form of glucose by capillary electrophoresis-diode array detector (CE-DAD) after enzymatic digestion (see method).
Primary examples in vaccine design have shown good levels of carbohydrate-specific antibody generation when raised using extracted or fully synthetic capsular polysaccharide glycans covalently coupled to a protein carrier.
Genomic DNA was extracted from fully expanded young leaves using a DNeasy Plant Mini Kit (Qiagen, Hilden, The quantity and quality of DNA were analyzed by NanoDrop™ ND-1000 spectrophotometer (Bio-Science, Budapest, Hungary).
