We have presented a brief example of how more information can be extracted from the type of published data that form a common source for demographic modelling.
The 12 pairs of VNTR-primers were tested using DNA extracted from the type strain (NCIMB 1947), five Norwegian, and five Chilean isolates of F. psychrophilum.
DNA extracted from the type strain of D. nodosus, CCUG 27824T (Culture Collection, University of Göteborg [CCUG], Sweden) was used as a positive control in the D. nodosus-specific real-time PCR and was included in each run.
mRNA was extracted from the different types of EB and it was noted that Gp130 and Stat3 (proteins involved in maintaining pluripotency) were upregulated only in the white KO population (data not shown).
This result is in line with the previously published results of F420 extracted from the wild type M. smegmatis cells having the major species of F420-5 F420-7 [9][11] [11], implying that the over expression of the FbiABC construct does not change the F420 production profile.
The transposable elements were extracted from the various types of masked repeats.
Total RNA was extracted from the wild-type and the hrpX mutant strains as described in our previous study [ 33].
For both C. elegans hybridizations, DNA extracted from the wild-type N2 strain has been used as the reference DNA.
Indirect enzyme-linked immunosorbent assay (ELISA) was performed using an outer membrane protein (OMP) fraction extracted from the wild-type SG strain, JOL420 [ 8].
In order to confirm this as a 5′-UTR extension, we performed RT-PCR using RNA extracted from the wild type and tfc6 mutants.
