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Viral RNA was extracted from the specimens using the QIAmp viral RNA extraction Kit (QIAGEN Ltd) with a starting sample volume of 140 μL and a final elution step with 60 μL of elution buffer.
Professor Starrs hopes to get some preliminary results on whether enough DNA can be extracted from the specimens for testing by Thanksgiving, he said, and it would take another month or more to get the DNA actually sequenced.
Finally, 8 mm diameter cylindrical cores were extracted from the specimens and their microarchitecture was reconstructed after synchrotron radiation microtomography experiments (isotropic resolution of 10 μm).
In spring 2014, all patients registered in the biobank with a diagnosis of CH were identified and DNA extracted from the specimens.
Total RNA was extracted from the specimens and amplified.
DNA will be extracted from the specimens for HPV testing by real-time PCR.
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Analysis of mitochondrial DNA extracted from the specimen showed that it was genetically similar to bears in southern Canada and the northern United States today.
The usual pyrosequencing approach, however, makes use of a first step in which the whole DNA extracted from the specimen is pyrosequenced and a second step in which, among the massive high-throughput outcome, only the endogenous DNA sequences are selected.
In this test Chlamydia antigen is extracted from the specimen by heating the swabs at 80°C with the Extraction Buffer.
Venom was extracted from the specimen using standard methods [ 79] (ketamine, 35 μg/g; pilocarpine, 6 μg/g).
Venom was extracted from the specimen (SVL/TL = 1,315/290 mm, weight = 298 g) as above but dosing with ketamine at 20 μg/g.
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