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To validate that microRNAs are indeed reproducibly detectable in serum, RNA was extracted from the sera of two healthy unrelated individuals and the levels of highly abundant microRNAs examined (Figure 2A).
DNA extracted from the sera and cervical smears from 5 patients with cervical abnormalities and 5 healthy women was serially diluted (in 10-fold steps) in distilled water and the presence of TTV DNA was detected by PCR NCR.
In an attempt to identify the cause of this increase, fucoproteins were extracted from the sera of cancer patients and healthy individuals using a fucose-specific lectin (lotus tetragonolobus) coupled to Sepharose, and were analysed by polyacrylamide gel electrophoresis and silver staining.
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MicroRNA was extracted from the pooled sera based on Toray's original protocol.
Their sizes and reactivity to silk specific sera matched proteins extracted from the skin (Figure 3).
Extracted from the Guardian, May 17 2008.
Nucleic acids were extracted from sera using the QIAmp MinElute Virus Spin Kit (QIAGEN, Santa Clarita, California, USA) following the manufacturer's instructions.
RNA was extracted from sera using the acid guanidinium-phenol-chloroform method.
Nucleic acid was extracted from patient sera using High Pure Viral Nucleic Acid Kit (Roche, Grenzach-Wyhlen, Germany) according to the manufacturer's instruction.
The levels of these 147 miRNAs were evaluated in RNAs extracted from all the training/testing sera (and also from all other sera used in this study).
RNA extracted from sera from dengue patients and from the corresponding DENV isolates were subjected to modified 3' RACE using oligonucleotide primers corresponding to the 3' and 5' ends of the DENV genome.
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