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By the late 1700s, most of our aristocratic homes would have been lit by a selection of candles made of expensive beeswax, or perhaps from even more expensive spermaceti, the wax extracted from the head cavities of sperm whales.
For genotyping, genomic DNA was extracted from the head (embryos) or tail (pups) and amplified by PCR according to the Jackson Lab protocol.
Data were extracted from the Head Injury Registry in Taiwan.
RNA was extracted from the head of single individuals as described above.
DNA was extracted from the head and thorax, using a CTAB-phenol/chloroform protocol [ 25].
Total RNA was extracted from the head kidney of both groups using Trizol reagent.
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Total RNA was extracted from the heads of wild type, TBPH minus alleles and the endogenous rescues (using D42-Gal4 and elav-Gal4) by using Trizol reagent (Invitrogen) as per the manufacturer's protocol.
Total RNA was extracted from the heads (Ambion, RNAqueous for PCR) immediately following decapitation.
RNA was extracted from the heads using the RNeasy Micro Kit (Qiagen, Antwerp, Belgium) according to instructions of the manufacturer.
Total RNA was extracted from the heads and bodies of male and female flies with a Trizol reagent (Gibco BRL).
Total RNA was extracted from the heads of individual bumble bees using a PicoPure kit (Molecular Devices, Sunnyvale, CA).
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