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In the event of gaps between detected LCBs, sequence for this region was extracted from the appropriate strain and compared to its counterpart via BLAST to confirm the findings from MAUVE.
For regions identified as being solely present in CA88, sequence was extracted from the appropriate positions within the pseudomolecule and queried against NCBI's nr database using the blastx algorithm.
To do this, total RNA was extracted from the appropriate larval stages using TRI reagent (Sigma Genosys Ltd., UK) as previously described [ 19, 27].
For the other species, gene sequences were extracted from the appropriate nucleotide fa files, and their structure from the relevant gff3 files.
Total RNA was extracted from the appropriate stages of amoebae using an RNeasy RNA Extraction Kit (Qiagen) and first strand cDNA synthesized with Superscript III Reverse Transcriptase (Life Technologies).
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After the whole blood spots have been dried, a disk is punched out from the center of the DBS, and PI are extracted from the disk with appropriate solvents and extracts are analyzed.
These 34 soft tissue sarcomas were extracted from the NCDB using the appropriate second and third editions of the WHO International Classification of Disease for Oncology (ICD-0-2/3) site (C40.0-C40.9, C41.0-C41.9) and histology codes.
The nanoparticles were then extracted from the dendrimer interior with an appropriate hydrophilic or hydrophobic thiol.
As in Drovandi et al. (2015), we argue that this can be obtained in big data settings through the use of an initial learning phase where data are extracted from the big dataset such that appropriate models for analysis can be explored and prior distributions of parameters can be formed.
In particular, curves often define the boundaries of anatomical features of interest, allowing the position of these to be identified and, if appropriate, extracted from the larger object for separate analysis.
If the information was not available from the abstract the appropriate information was extracted from the full text.
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