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Total cellular RNA was extracted by the methods described previously [ 16].
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Durum wheat grains for durum wheat starch extraction were bought from Millbo, Italy and extracted by the method described in Vansteelandt and Delcour (1999).
Then, the natural frequencies of asphalt track models are extracted by the method of linear perturbation.
Fatty acid composition was determined on the oils extracted by the method of Bligh and Dyer (1959).
Biflavonoid fractions of the coarsely ground root bark, stem bark and seed were respectively extracted by the method of Iwu et al. [3].
Phospholipid and triglyceride content: Lipids of synchronized young adult worms were extracted by the method of Bligh and Dyer [36].
Briefly, brain tissue samples were homogenized in 2∶1 chloroform:methanol containing 0.5% butylated hydroxytolulene and lipids were extracted by the method of Folch [58].
Total cellular lipids were extracted by the method of Bligh and Dyer [39] and separated by two-dimensional thin layer chromatography (I: chloroform/methanol/25% aqueous ammonium hydroxide, 90∶54∶7; II: chloroform/acetone/methanol/acetic acid/water, 50∶20∶10∶10∶5).
For triglyceride analysis, lipids of synchronized young adult worms were extracted by the method of Bligh and Dyer [29], and the triglyceride contents were measured using enzymatic kit (Triglyceride E-test, Wako Pure Chemical Ltd).
Genomic DNA was extracted by the method described previously [ 45].
Total lipids were extracted by the method of Folch [17].
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