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Based on the extracted brain surface, the mid-sagittal plane was determined.
The extracted brain tissue from mice injected with Apt-MNC was dehydrated in increasing alcohol concentrations, cleared in xylene, and embedded in paraffin.
Møller's co-author, Johannes Erritzøe, a taxidermist and ornithologist at the House of Bird Research in Christiansfeld, Denmark, autopsied each bird, noted its mass, and weighed its extracted brain.
Here, the threshold is an area (area: represents the number of pixels) of brain which was extracted in each step when the extracted brain area is greater than the threshold (th), it means that we obtained the brain without skull (status of convergence).
To investigate that question, principal investigator Kazuya Iwamoto, a neuroscientist; Kato; and their team at RIKEN extracted brain tissue of deceased people who had been diagnosed with schizophrenia as well as several other mental disorders, extracted DNA from their neurons, and compared it with that of healthy people.
One of the most serious challenges we encountered was in mapping extracted brain region mentions to standardized lexicons.
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To provide insights into the nature of the RNA toxicity, we extracted brain-enriched RNA from flies expressing a toxic CAG-repeat mRNA (CAG100) and a non-toxic interrupted CAA/G mRNA repeat (CAA/G105) for microarray analysis.
The extracted brains were sectioned at 50 μm in the coronal plane.
The SPM coregistration function provided a mapping of the extracted brains into the Montreal Neurological Institute (MNI) space to obtain AC-PC registration while maintaining the native space volumes.
We then extracted brains and dissected them on ice into hypothalamus (HYP), prefrontal cortex (PFC), hippocampus (HIPP), and adjacent parietal cortex (PCX).
The preprocessing step and extracting brain regions time series were done via SPM 12, DPARSF 4.3, and MATLAB codes.
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