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Exact(10)
Here, we attempted to establish species identity of small metazoans based on total (extractable) protein content using a simple protein extraction procedure.
Assuming that 40% of the dry mass is extractable protein this would correspond to about 75 nM per mg extracted protein and hour.
The proposed method was successfully applied to the determination of extractable protein in NR latex and medical latex gloves and proved to be a convenient, rapid and inexpensive method.
We propose that the extraordinary abundance (4% of the extractable protein in maize pollen) of this major pollen allergen is the result of selection for a trait that functions primarily in providing differential access to ovules.
Holzhauser and Vieths [ 7] also observed that the amount of extractable protein from hazelnut and peanut varied, depending on the type of nut and the roasting conditions.
However, to our knowledge, there has been no attempt to establish species identity of small metazoans based on total (extractable) protein content.
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Watermelon seeds are a byproduct of juice manufacture and contain good-quality extractable proteins.
Wheat endosperm is an allohexaploid tissue in which 80 85% of the extractable proteins are gliadins and glutenins.
The insoluble nuclear matrix proteins were separated from the high salt extractable proteins by centrifugation at 6000 rpm for 10 minutes.
Extractable proteins were divided into soluble and insoluble fractions for each collection [ 16] and subjected to high resolution LC-MS/MS analysis after tryptic digestion and SCX separation.
Adenoma tissue yielded three times the amount of acid-extractable protein g-1 wet wt and adenoma extracts consistently had significantly greater specific activity over a wide protein concentration range.
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