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Exact(5)
Spectrophotometric (Beckman Coulter DU 650, Beckman Coulter, Inc., Fullerton, CA, USA) measurements of the acetonic extract were made at three different wavelengths (470, 645, and 663 nm), and chlorophyll and carotenoids concentrations were calculated according to Wellburn [37].
All administrations (aspirin and extract) were made orally.
Further dilutions of fenugreek extract were made by mixing with RPMI medium.
After this period, filtration and concentration of the extract were made distilling all the solvent on a rotary evaporator under reduced pressure (40 rpm at 60°C) and then in a water bath at 60°C, obtaining the crude extracts.
Physiological salt solutions were prepared fresh in distilled water on the day of experiment, while stock solutions of all the drugs and extract were made in distilled water/saline and stored at −20°C and the dilutions were prepared fresh on the day of the experiment.
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Nuclear extract was made by a nuclear extraction kit from Marligen Bioscience (cat. #11906-100).
The final concentration of the extract was made at 0.5 mol/L and the extraction rates of the target analytes were compared, respectively.
The egg extract was made from uninfected CAMs, following the same extraction and purification procedure as the virus samples above.
The extract was made using 20 g of fresh leaves.
Yeast extract is made through autolysis (European Association for Specialty Yeast Products 2016), where grown yeast is heated to 45 55 °C.
The extract was made up to 2 mL with acetonitrile before GC injection.
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