Sentence examples for extract was spotted from inspiring English sources

Dichloromethane extract was spotted on silica gel 60 F254 TLC plate and was developed in a mixture of ethyl acetate methanol ammonia (35:10:5 v/v) as the mobile phase and scanned at 320 nm.

Chloroform extract was spotted on silica gel 60 F254 TLC plate and was developed in a mixture of n-butanol methanol ethyl acetate-6 M ammon-butanol methanol ethyls the mobile phase and scanned at 290 nM.

5 μL of each sample extract was spotted in triplicate.

Each organic extract was spotted in the solid phase (silica gel U60 plates) and its respective extraction solvent was used as mobile phase.

After the addition of 10 µl of Tris-buffered saline (20 mM Tris-HCl, 150 mM NaCl [pH 7.4]), each extract was spotted onto a nitrocellulose membrane using a BIO-Dot microfiltration apparatus Bio-Rad Laboratoriess, Inc., Hercules, CA).

To monitor the extent of inactivation of the antibiotics, 10 µl of the reaction mixture (with and without the enzyme extract) was spotted on filter paper disks, which were placed on Mueller-Hinton agar plates seeded with a sensitive standard Pseudomonas aeruginosa PAOI strain.

To generate MALDI-TOF main spectra (MSP), eight replicates of a fungal protein extract were spotted and each spot was measured three times.

Small aliquots of the extract were spotted directly onto a silica (Kieselgel F-254, Merck, Darmstadt, Germany) plate that was developed in ethylacetate :methanol : ammonia (85 : 10 : 5) followed by 0.5% ninhydrin solution and developed to detect the presence of cathinone and cathine (Zhou and Ninghua, 2000).

Supernatants were vacuum-dried at 65 °C and dried pellets dissolved in 1 μl 80% methanol mg−1 fresh weight. 1 μl of methanolic extracts were spotted onto HPTLC silica gel 60 glass plates (Millipore).

The extracts were spotted and run it using the same solvent as mobile phase.

Extracts were spotted onto C18 reverse-phase thin-layer chromatography (TLC) plates (Mallinckrodt Baker, Philipsburg, NJ, USA) and developed with 60% methanol as the mobile phase.