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Standardization of each herb extract was performed by high performance liquid chromatography (HPLC) analysis.
A phytochemical analysis of the crude extract was performed by High Performance Liquid Chromatography coupled with a photodiode array and mass spectrometry (HPLC-PDA-ESI-qMS).
The extraction of phenolics compounds from Cymbopogon citratus extract was performed using C18 cartridge.
An extract was performed.
Phytochemical analysis of the plant extract was performed using various chemical tests.
Bioactivity-guided fractionation of the 70% aqueous ethanol extract was performed to identify the active compounds.
Synthesis of AgNPs using leaf extract was performed according to a previously described method [8].
The antibacterial activity of the plant extract was performed by the well accepted Bauer-Kirby disc diffusion method [27, 28].
To evaluate the role of V. oleifera in the AuNP synthesis, the electrooxidation of the V. oleifera extract was performed.
GC MS analysis of the plant extract was performed using a Perkin Elmer Clarus 500 system comprising an AOC-20i auto-sampler.
Incubation of L. johnsonii with green coffee extract was performed at 30°C (red diamond symbol), 37°C (green circle), and 50°C.
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