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Nuclear extract was made by a nuclear extraction kit from Marligen Bioscience (cat. #11906-100).
The final concentration of the extract was made at 0.5 mol/L and the extraction rates of the target analytes were compared, respectively.
The egg extract was made from uninfected CAMs, following the same extraction and purification procedure as the virus samples above.
The extract was made using 20 g of fresh leaves.
The extract was made up to 2 mL with acetonitrile before GC injection.
The methanolic seed extract was made in between 0.1 and 2.0 mg/ml.
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Yeast extract is made through autolysis (European Association for Specialty Yeast Products 2016), where grown yeast is heated to 45 55 °C.
Spectrophotometric (Beckman Coulter DU 650, Beckman Coulter, Inc., Fullerton, CA, USA) measurements of the acetonic extract were made at three different wavelengths (470, 645, and 663 nm), and chlorophyll and carotenoids concentrations were calculated according to Wellburn [37].
All administrations (aspirin and extract) were made orally.
The quantification of antioxidant in the extract is made by calculating the IC50 value.
Further dilutions of fenugreek extract were made by mixing with RPMI medium.
More suggestions(17)
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