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The aqueous extract was held at -80°C until it was dried in a vacuum centrifuge at ambient temperature.
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Samples for DNA extraction were held at −80 °C until DNA was extracted.
More importantly, we observed that free VP dye extracted from visudyne was held in Sephedex G-50 spin columns, whereas visudyne passed the spin columns into collection tubes, indicating that the extraction of VP made the downstream separation of free dye from the conjugated dye possible by Sephedex G-50 spin columns.
When using the extracted VP, free VP was held in the size exclusion Sephadex G50 spin columns when PBS buffer alone was added to the EDC-activated VP reaction (PBS-VP control in Figure 1C).
In order to obtain the hydroalcoholic extract (HAE) the plant material was held under controlled-temperature kiln drying (38°C), with EtOH mixture (70%): H2O at room temperature (25 30°C) for 72 hours.
Mr. Zawahri's lawyer, Nizar al-Ghurab, said his client was held primarily to "extract information from him about his brother".
The extract was incubated overnight in a holding tank with continuous stirring at 4°C with the addition of 10 mg/mL of bentonite and 0.1 M MgCl2.
He was held on his own for six weeks as his kidnappers tried to extract a ransom.
After compression, the plunger was held in place for 10 and 30 s, before the compacted forage was extracted.
An extract was performed.
The extract was tantalisingly brief.
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