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Exact(19)
Partial purification or separation of crude methanol extract was done by solvent solvent extraction.
In case of color formation, clean-up of the extract was done over florisil and eluted with 2% diethyl ether n-hexane.
Qualitative analysis of various phytocompounds present in the G. zeylanica aqueous extract was done using previously described protocol by Krishnamoorty et al. [12].
Cleanup of the extract was done over florisil (magnesium silicate, mesh 60 100, active at 1250 °F, Janssen Chimica) packed with anhydrous sodium sulfate at the top of the column.
Surface modification of 21 nm TiO2 NPs (Sigma Aldrich) with G. zeylanica aqueous extract was done by refluxing 25 ml of G. zeylanica aqueous extract with 0.30 g of TiO2 (mainly anatase).
Chemical profiling of the extract was done by HPTLC method.
Similar(41)
Phytochemical screening and standardization of the extract were done before commencement of the in vivo study.
Antioxidant activity of pigment extracts was done by measuring inhibitory concentration (IC50) of pigment extract against 2,2-diphenyl-1-picrylhydrazyl (DPPH) solution.
Moreover, a phytochemical screening of the methanolic extracts was done.
The total phenolic content of extracts was done according to a previous method [ 19].
The antifungal bioassay of extracts was done by hanging drop technique.
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