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The extract was decanted.
After stirring using a VWR Analog Vortex mixer for 35 min, the extract was decanted into another vial.
The reddish brown color extract was decanted after centrifuging at 200 rpm for 30 min to separate any un-dissolved ingredients.
The extract was decanted into a fresh glass vial and re-extracted with a fresh batch of methanol.
The extract was decanted into a clean conical tube and stored at -20°C until analysis up to 1 mo.
The petroleum ether carotenoid extract was decanted and dried under nitrogen gas and re-dissolved in 3 mL of mobile phase for HPLC analysis (82 : 18, hexane : acetone).
Similar(50)
After extraction, all seed extracts were decanted and filtered over a 0.45-μm syringe filter.
The TPH in the treatments was determined spectrophotometrically following the methods of Mishra et al.[13] and Vu-Duc et al.[59] for 30 min. Thereafter, the mixtures were allowed to settle, and extracts were decanted into a volumetric flask and plug.
The ether extracts were decanted once the aqueous phase was frozen in dry-ice and discarded.
Extracts were decanted then filtered using filter funnels fitted with Whatman No 1 filter papers.
The aqueous phase was frozen in a mixture of solid carbon dioxide and ethanol and the extracts were decanted into glass tubes and evaporated to dryness.
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