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Analytes were isolated through supported liquid extraction, and the final extract was analyzed via HPLC with MS/MS detection.
The crude extract was analyzed by HPLC and LC-MS.
The extract was analyzed by gas chromatography with nitrogen phosphorous detection.
In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7).
The final extract was analyzed by ultra-high performance liquid chromatography coupled with tandem mass spectrometry.
After removing excess TFA with NaOH (0.1 M), the extract was analyzed by HPLC with UV detection at 296 nm.
Then, the extract was analyzed using optimized maltodextrin (MD) modified CE method for separation of TOL enantiomers.
The PCR products were analyzed on an ABI Prism 310 capillary electrophoresis unit, as described.32 The areas under peaks 2 5 after the primer dimer were added and divided by the area under the ITAS peak (internal amplification standard).32 For semiquantitative measurement, a dilution series of HL60 extract was analyzed in parallel.
Every extract was analyzed in duplicate.
The extract was analyzed for nicotine by LC.
The extract was analyzed by HPLC using Chiracel OD12 RH column (Diacel, Japan).
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