Exact(3)
The carrier RNA from the kit was applied to extract virus nucleic acid with low molecular weight.
We used the QIAamp UltraSens Virus DNA kit (QIAGEN, Valencia, CA, USA) to extract virus DNA according to the manufacturer's instructions.
To establish the efficacy of the antiviral activity of MBS extract, virus yield reduction assay was performed in which plant extract was added to host cells (Vero and MRC-5) after infection with RSV and HSV-1, respectively.
Similar(57)
The extracted virus is purified, inactivated and treated to produce a vaccine.
The text search enables extracting virus information using any querying keywords.
The reductions of virus titers (RSV and HSV-1) were determined in term of virus titer log after treatment in addition to the reduction factor (RF), i.e. the ratio of the virus titer in the absence of the extract over virus titer in the presence of the extract [ 8].
The study included incubation of the extract with virus specific cells (Vero and MRC-5) or with the virus itself.
In order to evaluate the prophylactic activity of MBS extract on virus specific cells, non cytotoxic extract serial dilutions were incubated with the cells before the virus addition.
The filtrate was centrifuged at 8000 g and the supernatant liquid was the crude extract of virus.
MBS extract reduced virus titer in one to two logs.
To our knowledge, this study is the first human trial to address the effect of Spirulina platensis whole unfractionated dried extract on virus load, liver function, health related quality of life and sexual functions in patients with chronic HCV.
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