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In the present work, we use the X-ray structures to extract, via in silico high-throughput docking, compounds from proprietary and commercial databases and demonstrate the successful identification of active compounds by radioligand binding.
The corrosion products of JDBM do not cause significant adverse effect on the cell viability and growth during in vitro cytotoxicity test of the Mg extract via human vascular endothelial cells (HUVECs).
The high mass sensitivity of the 1.7 mm cryogenically cooled NMR probe allowed for the first time acquisition of direct detected 13C NMR spectra of fungal metabolites, i.e., dechlorogriseofulvin and griseofulvin, directly from crude extract via HPLC HRMS SPE NMR.
Comparison of the recombinant HM with a mixture of three recombinant allergens, as well as natural allergens in the whole C. album pollen extract via immunological experiments revealed that it has a much lower potential of IgE reactivity.
Chemical composition of the optimized extract via GC/MS indicated the presence of four major phenolic compounds, hydroxychavicol, eugenol, isoeugenol and 4-allyl-1,2-diacetoxybenzene with peak areas of 66.55, 11.92, 2.90 and 3.21% respectively.
In detail, the mechanism underlying the bioreduction of silver was hypothesized to first involve trapping of silver ions on the surface of proteins in the extract via electrostatic interactions (i.e., recognition process).
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N. oculata was rich in eicosapentaenoic acid (EPA) when extracted via ethanol extraction (36 ± 4 mass%) compared to low yields from n-hexane extraction (6.1 ± 0.3 mass%).
DNA was extracted via phenol extraction and ethanol precipitation.
Hsiao (18 g) was extracted via circumfluence extraction with 60%% ethanol for 1.5 h, and was then filtered.
A total of nine matching points from each of the seven calibration boards were extracted via the manual extraction method.
Groundwater samples for DNA sequence analysis were extracted via phenol chloroform DNA extraction (Sambrook & Russell, 2001).
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CEO of Professional Science Editing for Scientists @ prosciediting.com