Sentence examples for extract using the from inspiring English sources
Total genomic DNA was extracted from the pellets obtained by centrifugation of either cytosol or Triton extract using the QIAamp DNA extraction minikit (ref 51304, Qiagen, Hilden, Germany) according to the manufacturer's protocol.
After each extraction cycle, total sugars were assayed in the extract using the phenol-sulfuric acid assay.
The extract that presented higher antioxidant activity was obtained at 40 °C and 350 bar with 12.7 mmol L−1 Trolox/g of extract using the DPPH radical scavenging method and 61.3 mmol L−1 Trolox/g of extract using the rubrene singlet oxygen quenching method.
Finally, high binding affinity and specificity of the peptide ligand are confirmed by the purification of t-PA from crude porcine heart extract using the immobilized-ligand column for affinity chromatography.
The design of selective on-line focusing strategies in CE is highlighted by the analysis of microgram amounts of ethynyl estradiol derived from a female contraceptive pill extract using the dynamic pH junction method, which resulted in over a 100-fold enhancement in concentration sensitivity.
Here we describe the design of a specific linear RT primer for the reverse transcription reaction and a pair of PCR primers to be used in the endpoint PCR reaction for each miRNA marker, and presented a strategy for co-analysis of a set of miRNAs and DNA on the same extract using the same detection platform.
