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Aqueous metabolites were separated from the intracellular extract using a 2 : 1 : 3 chloroform:methanol:water extraction method.
Samples were processed by organic phase extraction in hexane and retinol was isolated from the extract using a C18 column (150 mm × 4.6 mm, 3 μm particle size) with a mobile phase mixture of acetonitrile, tetrahydrofuran, methanol and 1% ammonium acetate solution (68 22 7 3) flowing at 1 ml/min.
Chemical screening was performed on aqueous suspensions and organic solutions for each extract, using a well-known protocol [12, 27].
Twenty brine shrimp larvae were placed in vials containing extract using a plastic pipette with a 2 mm diameter tip.
This study examined the precipitation of curcuminoids from an ethanolic extract using a supercritical antisolvent process (SAS).
The H2O2 consuming and producing capacity were measured in compost extract using a Hydrogen Peroxide Assay Kit (BioVision, Milpitas, CA, USA).
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Proteins were extracted using a phenol extraction procedure [ 54].
Data were extracted using a predefined extraction form.
Plasmids were extracted using a plasmid extraction kit (QIAgen).
DNA was extracted using a DNA extraction kit (DNeasy, Qiagen).
Genomic DNA was extracted using a DNeasy extraction kit (Qiagen).
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