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Thousand units of E. coli β‐glucuronidase was added to the tissue extract to release 20‐HETE from conjugation with glucuronide.
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The significant difference in the lower anterior crowding could be explained by the fact that first premolars are extracted to release the severe crowding, whereas second premolars are extracted when the crowding is not severe to correct the class II molar relationship [[13]].
As described previously [ 37], the cellulase activity was measured by monitoring the ability of protein extracts to release fluorescent 4-methylumbelliferone (4-Mu) from 0.91 mM 4-methylumbelliferyl-β-D-lactopyranoside (MUL) [ 61] in the presence of 13.46 mM δ-gluconolactone after 20 min at 40°C and pH 4.75, relative to a 4-Mu standard curve.
The possibilities remain that the elicitor activity could be increased by heating in acid conditions or by treating the extract with enzymes to release increased quantities of biologically active oligosaccharides.
Yoo et al. first discovered in Xenopus egg extracts that Plk1 phosphorylates Claspin after prolonged interphase arrest in aphidicolin-treated extracts, leading to release of Claspin from chromatin and checkpoint inactivation [ 33].
We therefore utilised a protocol first extracting in CHAPS to release DG from the membrane, followed by a triton extraction step to precipitate the cytoskeleton and associated proteins in the triton insoluble fraction.
The purpose of this study was to investigate the ability of a biomedical hydrogel to extract and release these proteins from smashed dentin.
The most abundant in vivo protein DNA data type as of 2013 is ChIP-chip, in which proteins are cross-linked to DNA, cells or extracts are sonicated to release fragmented chromatin (typically a few hundred base pairs associated with proteins), the protein of interest is affinity purified, and the associated DNA is quantified using microarrays.
The complete nuclear matrix was further extracted with 2M NaCl to release the outer nuclear matrix proteins, and in some cases followed by digestion with DNase-free RNase A to remove the core filaments of the matrix.
Oil is extracted to be burnt; burning it releases greenhouse gases; maximising recovery means maximising greenhouse gases.
The resulting pellet was then extracted with 1.6 M NaCl to release the histones and other high salt soluble proteins.
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